Xenbase Image ID: 173188

Fig. 3. Hes3 represses NPB fates. (A) Unilateral injection of hes3GR mRNA (500 pg) at the 2-cell stage followed by Dex treatment at the gastrula stage (NF stage 10.5) blocked snai2, sox10, foxd3 and dmtra1 expression, while the expression domain of pax3 and zic1 was expanded. In these embryos, the neural plate genes sox2 and sox3 were also expanded (red line). (B) Injection of hes3GR mRNA in the absence of Dex had not effect on snai2 and sox10 expression. (A-B) Dorsal views, anterior to top. Injected side is to the right. The frequency of the phenotypes shown (%) and the number of embryos analyzed (n) are indicated in the upper right corner. (C) qRT-PCR analyses indicate that Hes3GR blocks NC induction (snai2 and sox10) by Wnt8 and Noggin in animal cap explants, and promote neural plate fate (sox2). (D) Similarly, Hes3GR blocks PPR gene induction (six1 and dmtra1) by Noggin in animal cap explants. Values are normalized to ef1α and presented as mean  ±  s.e.m. Statistically significant changes are indicated; (*) p < 0.05 and (**) p < 0.01 (Student's t-test), from three independent samples. Image published in: Hong CS and Saint-Jeannet JP (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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