Xenbase Image ID: 173212

Fig. 5: Nosip deficient Xenopus embryos show disturbed dorso-ventral patterning in the retina. A Schematic illustration of the measurement and results shown in B and C. The retina is circled. A first dashed line defines the dorso-ventral eye midline. A second dashed line from the nasal to the temporal side of the eyes was set perpendicular to the dorso-ventral midline. A third, red line was used to highlight the border of marker gene expression.  defines the angle of the dorso-ventral shift in gene expression of tbx3 and vax2. B and C tbx3 (B) and vax2 (C) expression by WMISH at stage 32 in the eye. Close-up views are shown. In contrast to Control MO injection, Nosip MO injection leads to a significant shift in dorso-ventral patterning of the retina. Quantitative evaluations are shown. Abbreviations: n, number of independent analysed embryos, n.s., not significant. Error bars visualize standard error of the means (s. e. m.); *** p≤0.001. P-values are calculated by a nonparametric, one-tailed Mann-Whitney rank sum test. Image published in: Flach H et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue tbx3.L tbx3-a, tbx3-b, Xltbx3, xtbx3 X. laevis Throughout NF stage 32 retina branchial arch dorsal vax2.L dres93, vax2-a, vax2-b, vax3, xvax2 X. laevis Throughout NF stage 32 ventral retina Image source: Published Larger Image Printer Friendly View

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