Xenbase Image ID: 173215

Fig. 8: Loss of Nosip results in reduced marker gene expression during anterior neural crest cell development. A Expression of twist and snai2 upon Control or Nosip MO injection at stage 14. Anterior views are shown. Neural crest induction at the lateral neural plate borders is affected (arrowheads). B Statistical quantification of data in A. C egr2 and twist expression upon Control or Nosip MO injection at stage 23 with reduced neural crest migration after Nosip depletion (arrowheads). Control MO injected embryos are unaffected. Lateral views are shown. D Statistical quantification of data in C. E Measurement of egr2 expression in the ventrally migrating neural crest cells demonstrate that Nosip deficiency results in a inhibited neural crest migration. Lateral views are shown. F Statistical quantification of data in E. Single embryos of one experiment with a representative phenotype were evaluated. Abbreviations: N, number of evaluated embryos in total; n, number of independent experiments. Error bars visualize standard error of the means (s. e. m.); * p≤0.05; ** p≤0.01; *** p≤0.001. P-values are calculated by a nonparametric, one-tailed Mann-Whitney rank sum test. Image published in: Flach H et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 14 cranial neural crest neural plate border snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 14 cranial neural crest pre-chordal neural plate border egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 23 rhombomere R3 rhombomere R5 anterior branchial crest migratory neural crest cell twist1.L twist, twist1-a, twist1-b, X-twi, Xtwi, Xtwist X. laevis Throughout NF stage 23 cranial neural crest migratory neural crest cell Image source: Published Larger Image Printer Friendly View

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