Xenbase Image ID: 173245

Figure 4. Disruption of p2ry4 reduced expression of head organizer genes. Uninjected (a, d, g, j, m, p), TALEN‐L injected (b, e, h, k, n, q), and TALEN‐L/R injected (c, f, i, l, o, r) embryos were subjected to WISH for dkk1 (a–f), cerberus (g–l), and chordin (m–r) at gastrula stage. The expression of dkk1 and cerberus was reduced in TALEN‐L/R injected embryos at mid‐gastrula stage (f, l, brackets). The change of chordin expression in TALEN‐L/R injected embryos was not significant (o, r) compared to that in uninjected and TALEN‐L injected embryos (m, n, p, q). Dorsal halves of sagittally fractured embryos are shown. Dashed lines indicate the edge of blastocoel. Brackets indicate the area that were reduced in the expression of dkk1 and cerberus. Arrowheads indicate blastopore Image published in: Harata A et al. (2019) © 2018 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue dkk1.L dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Throughout NF stage 10.5 dorsal marginal zone dkk1.L dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. laevis Throughout NF stage 11 dorsal marginal zone cer1.L cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 X. laevis Throughout NF stage 10.5 dorsal marginal zone cer1.L cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 X. laevis Throughout NF stage 11 dorsal marginal zone chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10.5 dorsal marginal zone chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 11 dorsal marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + p2ry4 TALEN + NF11 (in situ hybridization) Fig. 4 f Expression Phenotype decreased amount dkk1.L expression in dorsal marginal zone Xla Wt + p2ry4 TALEN + NF11 (in situ hybridization) Fig. 4 l Expression Phenotype decreased amount cer1.L expression in dorsal marginal zone Larger Image Printer Friendly View

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