Xenbase Image ID: 173246

Figure 5. p2ry4 resulted in ectopic expression of head organizer genes. No injection (a, d, g, j) and injection of 500 pg of p2ry4 mRNA (b, e, h, k) and 1,000 pg of p2ry4 mRNA (c, f, i, l) into the ventral side at 8‐cell stage and collection at blastula stage (a–c). Embryos were hemisectioned and subjected to WISH for dkk1 (d–f), cerberus (g–i), and chordin (j–l). Overexpression of p2ry4 led to the accumulation of pigment (b, c, arrows). Dkk1 or cerberus expression was induced by p2ry4 mRNA (e, f, h, i, arrowheads). Animal views (a–c). Arrowheads indicate injected sides Image published in: Harata A et al. (2019) © 2018 The Authors. Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 9 dorsal marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + p2ry4 + NF9 (whole-mount microscopy) Fig. 5 b c Anatomical Phenotype increased pigmentation in the animal hemisphere Xla Wt + p2ry4 + NF9 (in situ hybridization) Fig. 5 e f Expression Phenotype mislocalised dkk1.L expression in ventral marginal zone Xla Wt + p2ry4 + NF9 (in situ hybridization) Fig. 5 h i Expression Phenotype mislocalised cer1.L expression in ventral marginal zone Larger Image Printer Friendly View

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