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Figure 2. Identification of Xbra response elements (XbRE) within the ventx1.1 promoter region. Different serially-deleted ventx1.1 promoter constructs were co-injected either with or without DN-Xbra or Xbra at the 1-cell stage and grown until stage 11 in 30% MMR to measure the relative promoter activity at the stage 11. (a) ventx1.1 (−2525) promoter injected either with or without DN-Xbra. (b) ventx1.1 (−2525) promoter injected either with or without Xbra. (c,d) Serially-deleted promoter constructs of ventx1.1 injected either with or without Xbra. (e) ventx1.1 (−103) promoter construct injected either with or without Xbra in a dose-dependent manner. (f) Putative Xbra binding consensus was mutated by site-directed mutagenesis within ventx1.1 (−103) promoter constructs. (g) XbRE-mutated ventx1.1 (−103) mXbRE and ventx1.1 (−103) promoter constructs injected either with or without Xbra. (h) ventx1.1 (−103) promoter construct injected either with or without FoxD5b. (i,j) A ChIP-PCR assay was performed with anti-Myc antibody during gastrula. All ChIP bindings were measured by PCR using specific primers. ventx1.1 (−103) promoter DNA was used as a positive control while Xvent2 coding region primers were used as a negative control for all the ChIP-PCR experiments. All the relative promoter activity data are shown as mean ± SE. The ChIP-PCR band intensities were quantified using a densitometer. Image published in: Kumar S et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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