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Figure 4. Xbra enhances Smad-1 binding affinity on its cis-acting element (BRE) within the ventx1.1 promoter. To perform ChIP-PCR assays, mRNA was co-injected either with or without Smad-1 at the 1-cell stage and harvested the embryos until stage 11 in 30% MMR. (a,b) The ChIP-PCR assay was performed with the anti-Myc antibody. (c,d) The ChIP-PCR assay performed with the anti-Flag antibody at the stage 11. All bindings were measured by PCR method using specific primers. ventx1.1 (−103 and −180) promoter DNA served as positive control. The ChIP-PCR band intensity was quantified using a densitometer. Image published in: Kumar S et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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