Xenbase Image ID: 173259

Figure S1. Schematic of epithelial marker apical intensity calculations. Related to Figure 1, Figure 2, Figure 5 and Figure 6. (A) Transverse sections of stage 28 control embryo stained for tropomyosin (red) and ZO-1 (green). To calculate apical intensity, the red and green channels are first split. The tropomyosin channel is used to segment out and create a mask for cardiomyocytes. The ZO-1 channel has intensity normalized to background signal in the endoderm and undergoes a small Gaussian blur (kernel = 2 pixels). (B) Applying the mask to the normalized ZO-1 channel generates an image with only ZO-1 expression in cardiomyocytes. A 10-pixel wide linear region around along the apical surface is created to measure apical intensity. Scale bar = 50 μm. (C) 3D projection of the ventral, cardiomyocyte-masked, ZO-1 expression, showing apical intensity measurements at 4 different z-locations. Image published in: Jackson TR et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tpm1.L alpha-tm, alpha-tropomyosin, CH1 X. laevis Throughout NF stage 28 mesoderm cardiac myocyte tjp1.L zo-1, zo1 X. laevis Throughout NF stage 28 ectoderm mesoderm cardiac myocyte Image source: Published Larger Image Printer Friendly View

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