Xenbase Image ID: 173263

Figure S5. Targeted injections to perturb HPC contractility shows cell-autonomous mechanics influence MET. Related to Figure 5. (A) Transverse sections of stage 28 embryos stained for tropomyosin (red) and ZO-1 (green) with rhodamine dextran (RDA; cyan) showing expression of construct target injected into HPCs. Expression of arhgef2-C55R results in visibly elevated expression of aPKC (arrow). Quantification of individual cell apical intensities of RDA and aPKC show significantly correlation in arhgef2-C55R expressing HPCs but not RDA controls or embryos expressing arhgef2-C55R and treated with 50μM Y27632. Scale bar is 50 μm. (B) Stage 39 embryos with HPCtargeted expression of injected constructs. arhgef2-C55R embryos show relatively normal hearts with defects localized to where arhgef2-C55R is expressed. Treatment with Y27632 partially rescues these defects. Scale bar = 100 μm. Image published in: Jackson TR et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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