Xenbase Image ID: 173273

Fig. 3. Sox5 antagonizes the inhibitory effect of Tcf15 and Meox2 in Myod1-induced myogenic transcription assay. (A) Embryos were injected unilaterally either with Myod1 synthetic mRNA (100 pg) alone or co-injected with Tcf15, Tcf15 plus Sox5, Meox2, Meox2 plus Sox5, Pax3 or Pax3 plus Sox5 (400 pg each), fixed at neurula stage and probed with Des and Actc1. (B) Same experiments here as in (A) on animal cap explants. The arrowhead indicates ectopic expression. Probe is shown in the bottom right corner of each panel. (C) Bar graph showing percentage of embryos with no, faint or strong ectopic expression. Chi-squared test analysis for ectopic Des expression: Myod1 versus Sox5 (P = 0,0008), Tcf15 (P value< 0,0001), Meox2 (P value< 0,0001), Pax3 (P value< 0,0001), Myod1+Tcf15 versus Myod1+Tcf15+Sox5 (P value< 0,0001), Myod1+Meox2 versus Myod1+Meox2+Sox5(P value< 0,0001), Myod1+Pax3 versus Myod1+Pax3+Sox5 ns, for ectopic Actc1 expression: Myod1 versus Sox5 (P = 0,0207), Tcf15 (P value< 0,0001), Meox2 (P value = 0,0040), Pax3 (P value = 0,0002), Myod1+Tcf15 versus Myod1+Tcf15+Sox5 (P value = 0,0008), Myod1+Meox2 versus Myod1+Meox2+Sox5 (P value = 0,0039), Myod1+Pax3 versus Myod1+Pax3+Sox5 ns, for ectopic Des expression in animal caps: Myod1 versus Sox5 (P = 0,0228), Tcf15 (P value = 0,0350), Meox2 (P value< 0,0001), Pax3 (P value = 0,0056), Myod1+Tcf15 versus Myod1+Tcf15+Sox5 (P value< 0,0001, Myod1+Meox2 versus Myod1+Meox2+Sox5 (P value< 0,0001), Myod1+Pax3 versus Myod1+Pax3+Sox5 ns, for ectopic Actc1 expression in animal caps: Myod1 versus Sox5 (P = 0,0003), Tcf15 (P value = 0,0014), Meox2 (P value = 0,0069), Pax3 (P value< 0,0001), Myod1+Tcf15 versus Myod1+Tcf15+Sox5 (P value< 0,0001), Myod1+Meox2 versus Myod1+Meox2+Sox5 (P value< 0,0001), Myod1+Pax3 versus Myod1+Pax3+Sox5 ns. Numbers of injected embryos are indicated at the bottom of each bar. Image published in: Della Gaspera B et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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