Xenbase Image ID: 173275

Fig. 5. DXM-inducible proteins have the same activities as native proteins in Myod1-induced myogenic transcription assay. (A) Induction by 10 µM dexamethasone (DXM) at morula stage, led to strong ectopic expression of Des in embryos injected unilaterally by Myod1GR (100 pg). (B) Inducible proteins GAMeox2, GASox5 and GASox5-A triggered strong ectopic expression of Des like native ones in Myod1-induced myogenic transcription assay, after unilateral co-injection of the corresponding synthetic mRNA (400 pg) with Myod1 (100 pg) and induction by 10 µM DXM. (C) Bar graph showing percentage of embryos with no, faint or strong expression. Chi-squared test analysis for Des expression: Myod1GR no DXM versus Myod1GR DXM (P value< 0,0001), Myod1 versus Myod1+GAMeox2 ns, Myod1+GASox5 no DXM ns, Myod1+GASox5-A no DXM ns, Myod1+GAMeox2 no DXM versus Myod1+GAMeox2 DXM (P value = 0,0147), Myod1+GASox5 no DXM versus Myod1+GASox5 DXM (P value = 0,0298), Myod1+GASox-A no DXM versus Myod1+GASox5 DXM (P value = 0,0005). Numbers of injected embryos are indicated at the bottom of each bar. Image published in: Della Gaspera B et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue des.1.L csm1, csm2, des.1-a, des.1-b, desm X. laevis Throughout NF stage 13 to NF stage 21 somite presomitic mesoderm Image source: Published Larger Image Printer Friendly View

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