Xenbase Image ID: 173283

Fig. 2. Cdk-dependent phosphorylation of Otx2. (A) Cleavage arrest by HAcyclin B1* or HA-cyclin A1*. mRNA was injected into one blastomere at the twocell stage, and embryos were observed at the 32-cell (stage 6) or early blastula (stage 7) stage. Animal view. (B,C) Western blotting of Myc-Otx2δAD coexpressed with HA-cyclin B1* (B) or HA-cyclin A1* (C). Lysates were treated with λ-PP as indicated. (D) Phenotype of p27xic1-overexpressing embryos (left) and western blotting of Myc-Otx2δAD co-expressed with p27xic1 (middle, right). Co-injection (middle): Myc-Otx2δAD mRNA (500 pg/embryo) was coinjected with (lane 2) or without (lane 1) p27xic1 mRNA (2 ng/embryo) into one blastomere at the two-cell stage. Sequential injection (right): p27xic1 mRNA (1.5 ng/embryo) was injected into one blastomere at the two-cell stage, then mRNAs for Myc-Otx2δAD (500 pg/embryo) and p27xic1 (500 pg/embryo) were co-injected into the cleavage-arrested blastomeres at the 32-cell-stage equivalent (lane 4). Myc-Otx2δAD mRNA was injected at the two-cell stage as control (lane 3). Lysates were prepared from embryos at stage 8 (B), stage 9 (C and D, lanes 1 and 2) or stage 9.5 (D, lanes 3 and 4). White asterisks indicate cleavage-arrested blastomeres. Scale bars: 500 μm in A and D. Image published in: Satou Y et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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