Xenbase Image ID: 173284

Fig. 3. Eye phenotypes caused by Otx2 mutants. (A-D) Representative eye phenotypes. mRNAs for Otx2 construct and nbgal as a tracer were co-injected into the presumptive eye field of the dorsal-left blastomere at the four-cell stage. Phenotypes were scored at stages 38-42: normal indicates normallooking eyes; small/trace indicates small or trace eye; ventral defect indicates normal eye width with pigmentation defect on the ventral side; enlarged indicates enlarged eye. Some injected embryos showed gastrulation arrest, which were removed from the total scores. White bars indicate the diameter of the eye. Anterior to the left, dorsal is up. (E) Incidence of eye phenotypes. See A-D for color codes of phenotypes. mRNAs for Otx2 construct or globin (negative control) was injected at 20 or 40 pg/embryo as indicated. n indicates the total number of scored embryos. (F) Quantitative analysis of the eye size. **P<0.01 (Student’s t-test); error bars, s.e.m.; n indicates the number of samples. (G-H′) Transverse hemisection across the left eye. (G,G′) Control. (H,H′) Enlarged eye caused by Ox2-4A. (G,H) Bright field. (G′,H′) DAPI staining. Arrowheads indicate pigmentation defect in the ventral retina. (I) Incidence of eye phenotypes caused by Otx2-A2A constructs. In the A2A constructs, the remaining intact serine residue is indicated. Scale bars: 500 μm in A-D; 100 μm in G,H. Image published in: Satou Y et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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