XB-IMG-173289
Xenbase Image ID: 173289
|
Fig. S1. Post/translational modifications ofOtx2 aroundthe repressiondomain.
Western blot analysis of Myc@Otx2 constructs expressed in X. laevis embryos. (A)
Myc@Otx2 (FL) and its deletion constructs (HD, ΔAD, AD and ΔRD). Boxes indicate
the data presented in Fig. 1B. ΔRD appeared to have a modified band (orange
arrowhead). (B) WT and alanine mutants at S132 (S132A), S123 (S123A), S122
(S122A), S153 (S153A), S158 (S158A) and S161 (S161A) in Myc@Otx2ΔAD
constructs. (C) WT and alanine mutants at S116 (S116A), S132 (S132A), S158
(S158A) in Myc@Otx2ΔAD constructs. (D) λ@PP treatment removed most modified
bands of WT and 3Aconstructs of Myc@Otx2FL. Note that the different electrophoretic
mobility of nascent bands (blue arrowheads) between FL and FL@3A may be due to
substitutions of serine with alanine, though Myc@Otx2@ΔAD and ΔAD@3A migrated
similarly (see Fig. 1D). Calculated and apparent molecular masses of constructs
(kDa) were 43 and 50 for Myc@Otx2FL, 22 and 30 for Myc@Otx2@HD, 32 and 40 for
Myc@Otx2ΔAD, 23 and 35 for Myc@Otx2@AD, and 34 and 50 for Myc@Otx2ΔRD,
respectively. Black circles, undesired products or degradation productsY orange
arrowheads, bands resistant to λ@PP, which may correspond to an upper band of ΔRD
in panelA. Image published in: Satou Y et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |