XB-IMG-173300
Xenbase Image ID: 173300
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Fig. 1. Knockdown of ADAM13 or ADAM19 inhibits Wnt
signaling and NC specification but not NPB formation.
(A) cDNA sequences of adam12 and adam19 showing the
translation start sites (red) and targets of MOs (colored
boxes). (B) Knockdown (KD) of ADAM13 or ADAM19 inhibits
head cartilage formation. Transgenic X. tropicalis snail2-
eGFP embryos were injected in one anterodorsal (D1)
blastomere at the eight-cell stage with the indicated MO
(1.5 ng each) cultured to stage ∼46 and imaged for eGFP
expression. One representative embryo of each injected
group (ventral view) is shown in the upper panels and the
results of five independent experiments are shown in the
lower panel. (C,D) Effects of ADAM13 or ADAM19 KD on NC
and NPB marker expression. Wild-type embryos were
injected in one blastomere at the two-cell stage with the
indicated MO (6 ng each) and cultured to stage ∼12.5.
Embryos were processed for in situ hybridization for the
indicated genes. (E) KD of ADAM13 or ADAM19 inhibits
endogenous Wnt signaling. Transgenic Wnt reporter
embryos were injected as in C,D, cultured to stage ∼12.5 and
imaged for eGFP expression. White arrowheads indicate the
Wnt signal at the NPB. Embryos in C-E are shown in dorsal
view, with anterior at the top. Red asterisks indicate the
injected side. CT, control MO; n, number of embryos scored;
N, number of independent experiments performed;
***P<0.001; NS, not significant. Image published in: Li J et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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