Xenbase Image ID: 173301

Fig. 2. ADAM13 and ADAM19 function in NC specification through different mechanisms. Wild-type embryos were injected into one blastomere at the two-cell stage with the indicated MO (6 ng each) and mRNA (100 pg each), cultured to stage ∼12.5 and processed for in situ hybridization for the indicated genes. (A) Effects of ectopically expressed ADAM13 and ADAM19 variants on NC markers. (B) Both wild-type ADAM19 and the protease-dead E/A mutant rescue the NC specification defects caused by ADAM19 KD. Rescue constructs were generated as described in the Materials and Methods. All embryos are shown in dorsal view, with anterior at the top and a red asterisk denoting the injected side. U, uninjected; WT, wild type. ***P<0.001. Image published in: Li J et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 12.5 neural plate border sox9 cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. tropicalis Throughout NF stage 12.5 neural plate border Image source: Published Larger Image Printer Friendly View

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