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Fig. 3. ADAM19 controls ADAM13 protein levels in vivo. (A) Effects of various MOs on exogenously expressed ADAM13 and ADAM19. Embryos were injected in one blastomere at the two-cell stage with the indicated RNA (100 pg each) and MO (6 ng each), and cultured to stage ∼19. Whole-embryo lysates were processed for western blot (WB) for the C-terminal myc6 tag on the ADAM constructs. (B) Effects of various MOs on endogenous ADAM13. One-cell stage embryos were injected with the indicated MO (12 ng each) and cultured to stage ∼12.5. Embryo lysates were processed for western blot for endogenous ADAM13. (C) Upregulation of exogenous ADAM13 by coexpressed ADAM19. Embryos were injected with the indicated transcripts (100 pg each; the encoded ADAM13 was myc6-tagged and ADAM19 was HAtagged) and cultured as in A, and lysates were processed for western blot with an anti-myc antibody. Single and double arrowheads indicate the pro- and mature forms of ADAMs, respectively. Image published in: Li J et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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