Xenbase Image ID: 173305

Fig. 6. The stabilized ADAM13 mutants can rescue the NC specification defects caused by ADAM19 knockdown and induce ectopic snail2 in the ventral ectoderm. (A,B) Rescue of the reduced sox9 expression caused by ADAM19 knockdown (KD) (A) or ADAM13 KD (B) by various transcripts. Embryos were injected into one blastomere at the two-cell stage with the indicated RNA (100 pg each) and MO (6 ng each), cultured to stage ∼12.5 and processed for in situ hybridization for sox9. Embryos are shown in dorsal view, with anterior at the top and a red asterisk indicating the injected side. (C) Induction of ectopic snail2 by different variants of ADAM13. Embryos were injected into one posterior-ventral (V2) blastomere at the eight-cell stage with the indicated RNA (50 pg each) and MO (1.5 ng each), cultured to stage∼12.5, and processed for in situ hybridization for snail2. Only the injected side is shown, with anterior at the top and dorsal to the left. Arrows indicate the ectopic snail2 expression. (D) A model for the regulation of ADAM13 stability and NC specification by ADAM19. Crescents indicate the metalloproteinase domains of ADAMs. See Discussion for an explanation. ***P<0.001; NS, not significant. Image published in: Li J et al. (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue sox9 cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. tropicalis Throughout NF stage 12.5 neural plate border snai2 slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. tropicalis Throughout NF stage 12.5 neural plate border Image source: Published Larger Image Printer Friendly View

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