Xenbase Image ID: 173327

Figure 4. znf367 morphants analysis and control rescue experiments. (A) mRNA distribution of sox2 and rx1 in znf367 morphants and controls. (B) Statistical analysis of the data in A. (C) The levels of expression for the indicated mRNAs were evaluated for Co-MO or ZNF367-MO populations by qRT-PCR, and normalized to that of the housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (gapdh) expression, and the mean of the Control-MO was set to 1. For each gene, three independent RNA samples from morphants and controls were analysed. (D) The morphants phenotype can be rescued by the co-injection of morpholino plus full length Xenopus znf367 mRNA as shown by the recovered expression of sox2 and elrC. Abbreviations: n, number of independent experiments; N, number of evaluated embryos in total; Error bars indicate standard error of the means (s.e.m); *p ≤ 0,05; **p ≤ 0,01. P-value were calculated by Student’s t-test. Image published in: Naef V et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 14 neural plate pre-chordal neural plate chordal neural plate rax.L rax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1A X. laevis Throughout NF stage 14 optic field pre-chordal neural plate anterior neural fold elavl3.L elrC, HuC X. laevis Throughout NF stage 20 trigeminal placode neuron Rohon-Beard neuron interneuron Image source: Published Larger Image Printer Friendly View

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