XB-IMG-173338
Xenbase Image ID: 173338
|
Fig. 1. gli2 and Ptc1 expression patterns in Xenopus embryos. (A–G, J–K, M–O) Dorsal views of Xenopus laevis embryos, anterior side on the left. (L) Dorsal view, anterior side at the top. (J′, K′): Transversal sections, dorsal side at the top. (H–I) Lateral views, anterior side on the left. (K′) Anterior view. (A–B) gli2 is expressed in the dorsolateral region of the embryo at stages 12.5 and 13 (yellow arrowheads). (C–D) gli2 transcripts are detected in the NPB at early neurula stage (yellow arrowheads) and in the anterior NP (black arrow). No expression can be observed in the dorsal midline (red arrowhead) (E–G). From stage 17 to 19, gli2 becomes more prominent in the lateral and anterior NPB. (H–I) At tailbud stages, gli2 is expressed in the mandibular (mb) and hyoid (hy) NC streams, in the branchial arches (b.a.), in the otic (Ot) and optic (Op) vescicle and in the presomitic mesoderm (S). (J–K) Double ISH showing gli2 expression with two gene markers. (J–J') gli2 is expressed in an overlapping territory with the NC marker foxd3. (K–K′) The domain of gli2 expression extends laterally from the NP, as evidenced by the double ISH with sox2 in whole embryos. (K′) Anterior view showing that the expression of gli2 extends mores anteriorly than that of sox2. (L) At the late gastrula stage, Ptch1 is expressed in the dorsal side of the blastopore. (M–O) At neurula stages, Ptch1 can be detected in the NPB and in the neural folds. (O′) Expression of Ptch1 in the cell membranes of ectodermal cells. (P) Diagram representing the expression domains of the Ptch1 protein (green) and the genes gli2 (blue), foxd3 (light yellow) and sox2 (pink). Image published in: Cerrizuela S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
Image source: Published Larger Image Printer Friendly View |