Xenbase Image ID: 173340

Fig. 3. gli2 requirement in NC specification. (B–G, I–N, P–U) Dorsal views of Xenopus laevis embryos at mid-neurula stage; anterior side is on the left. Arrowheads indicate the injected side (turquoise). The expression of the genes is shown in purple. (A) Graphic representation of the experiments. (B–D) gli2MO-injected embryos show reduced expression of the NC markers foxd3 (B), snail2 (C) and sox9 (D). (E–G) Increase in the expression of the NP marker sox2 and the epidermal marker xk81a after gli2MO injection. Figure G shows a double ISH of sox2 and xk81a, with brackets indicating the width of the NC domain. (I–N) The opposite effect is seen when gli2 is overexpressed in the NC territory. (H, O) Quantification of gli2MO and of gli2 mRNA effects on the expression of NC markers, respectively. (P–Q) Overexpression of gli2 with the construct α3000gli2 produced an increase in the expression of foxd3 and snail2. (R–S) Control of morpholino specificity. Coinjection of gli2MO morpholino along with a plasmid encoding a mutated gli2 at the morpholino binding site (CRgli2) rescued the expression of the NC markers foxd3 and snail2. (T) The injection of the α3000CRgli2 construct also rescued foxd3 expression. (U) The microinjection of CoMO did not alter foxd3 expression. (V) Quantification of the experiments shown in Fig. 3P–U. Image published in: Cerrizuela S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Sometime during NF stage 15 to NF stage 16 neural crest snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Sometime during NF stage 15 to NF stage 16 neural crest sox9.L cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Sometime during NF stage 15 to NF stage 16 neural crest sox2.S anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Sometime during NF stage 15 to NF stage 16 epidermis Image source: Published Larger Image Printer Friendly View

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