XB-IMG-173342
Xenbase Image ID: 173342
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Fig. 5. The activator form of gli2 is required for NC specification. (A) Schematic procedure of the experiments. (B–K, M–P, R–T) Dorsal views of Xenopus laevis embryos at mid-neurula stage, anterior side to the left. Arrowheads indicate the injected side, shown in turquoise. (B–C) gli2act increases the expression of foxd3 and snail2. (D-G) gli2act and α3000gli2act are able to rescue the expression of foxd3 and snail2 in the morphant embryo. (H–K) gli2rep represses the expression of foxd3 and snail2 and therefore is not able to rescue these expressions in the morphant embryo. (L) Quantification of effects shown in B–K. (M–P) The phosphorylation mutant gli2PSM increases the expression of NC markers and represses the activity of sox2 and xk81a. (R-T) The α3000gli2PSM construct also increases the expression of foxd3 and is able to rescue the effects of gli2MO. (Q, U) Quantification of the experiments shown in M–P and R–T, respectively. Image published in: Cerrizuela S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
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