XB-IMG-173345
Xenbase Image ID: 173345
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Fig. 8. gli2 acts in a cell-autonomous manner in NC migration. (B–E, G, J–K, M–N, Q–S) Lateral views of embryos with the anterior side on the left showing the expression of the NC migration markers sox10 and foxd3. Figures Q–S show merged images of fluorescence and bright field captures. (A, F, I, L, P) Schematic representation of the different assays. (B) Stage 20 embryos showing a reduction in the migration front in the injected side evidenced by the expression of foxd3. (C) In stage 22 embryos the difference in expression between the control and the injected side is notably higher, as evidenced by the marker sox10. (D–E) Coinjection of either CRgli2 or gli2act with gli2MO rescued NC migration as shown by foxd3 expression. (F–G) Injection of GANT61 produced a loss of gli2 function and a noticeable decrease in NC cell migration, as shown by the expression of foxd3. (H) Quantification of phenotypes shown in B–G. (I–N) Implantation of GANT61-soaked beads into NC at two different time frames (stages 14–22 and 17–22) produced a reduction in the front of migration of the NC as shown by sox10 expression. (O) Quantification of experiments shown in Figures I–N. (P–S) gli2 acts cell-autonomously in NC migration. Grafting of uninjected NC explant into an uninjected wild type embryo produced no alteration in the front of migration of the NC. The injection of gli2MO in the transplanted explant altered the normal pattern of NC migration. Grafting of a wild type NC explant into a gli2MO injected host produced no changes in normal NC migration. Image published in: Cerrizuela S et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V.
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