Xenbase Image ID: 173479

Fig. 4 Overexpression of atf4 in Xenopus embryos did not cause apoptosis At stage 13.5, the TUNEL signals detected are very weak in control embryos, as well as in atf4GR injected embryos with or without dexamethasone treatment. (A−F) Representative embryos from each group with the strongest TUNEL signals. B, D, and F are higher magnification views of A, C, and E, respectively. Statistics are indicated in B, D, and F for each group. All the rest embryos analyzed in each group showed very weak signals (data not shown). Before TUNEL assay, the embryos were subjected to betagalactosidase staining to trace the injected sites. Image published in: Liu JT et al. (2011) Copyright © 2011. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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