XB-IMG-173487
Xenbase Image ID: 173487
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Fig. S1. (related to Fig. 1) Ectopic Ascl1 inhibits mesendoderm induction by VegT but not by
Nodal/Activin
(A) Representative control and ascl1 mRNA (500 pg)-injected embryos at stage 11 viewed from the
vegetal pole. (B) Embryos at stage 10.5 in situ hybridized with sox17α and viewed from the vegetal
pole. (C) β-gal or ascl1 mRNA (500 pg) was injected into the vegetal pole at two-cell stage and the
resultant embryos were collected at stages 6.5 and 8 for detecting maternal VegT mRNA by RTqPCR.
Values are mean ± s.d. (D) qPCR analysis for animal cap explants injected with indicated
mRNAs and cultured in the presence of CHX (100 mM) from stage 8-10.5. (E) mRNA encoding
Myc-tagged VegT (MT-VegT) was injected with or without Ascl1-HA at the two-cell stage, and the
resultant embryos at stage 8 were analyzed for the expression of MT-VegT protein by western
blotting. (F) Western blotting using anti-phospho-Smad2/3 (pSmad2/3) antibodies. Animal caps
dissected from control or ascl1-injected (1 ng) blastulae were treated with 5-50 ng/ml Activin protein
for 2 h followed by western blotting. (G) Animal caps isolated from blastulae injected with Xnr1 (100
pg) alone or together with ascl1 (500 pg) were analyzed by qPCR for mesendoderm gene expression.
Values are mean ± s.d. Image published in: Gao L et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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