XB-IMG-173488
Xenbase Image ID: 173488
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Fig. S2. (related to Fig.2) Ascl1 morpholinos and GO analysis for genes altered in Ascl1
morphants
(A) Schematic of the reporter constructs used to assess the translation blocking effects of the Ascl1
MOs: MOa, MOa2, and MOb. A Myc-tag (MT) is inserted in the C-terminus of Ascl1 polypetide. (B)
cDNA sequences flanking the start codon (red characters) of two pseudoalleles of Ascl1. The MO-recognizing sequences are indicated. (C) qPCR analysis of ascl1a and 1b expression in Ascl1
morphants at stage 10.5. Values are mean ± s.d. **P<0.01. (D) Western blot analysis of MOa (MOb)
inhibition of the translation of reporter Ascl1b (1a). (E) Representative control and Ascl1 morphants
at different development stages. Incidence of blastopore closure delay: 97.8% (n=184) in Ascl1
morphants. All control morphants (cMO) closed blastopores by the end of gastrulation (n=168). The
incidence body axis shortening with anteriorization of gut was near 100% (152/155) in Ascl1
morphants. The data were combined from 4 independent experiments. (F) Results from GO analysis
for the genes (1095 counts) altered by Ascl1 depletion in embryos at stage 10.5. Image published in: Gao L et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |