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Fig. S3. (related to Fig. 2) Effects of Ascl1 depletion on cell division and gene expression (A) 80 ng cMO or Ascl1 MOs injected at one-cell stage, RLDX injected into one animal-pole cell at 32-cs. Rhodamine channel (upper panels) or bright field (BR, lower panels) photographs taken at stage 10.5. (A’) Comparison of cell numbers in the RLDX-labeled clones from 10 control or Ascl1 morphants at stage 10.5. n.s.: no significance by Student’s t-test. (B) Gene expression in control and Ascl1 morphants during stage 9 to stage 11.3 analyzed through the semi-qPCR. Image published in: Gao L et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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