XB-IMG-173491
Xenbase Image ID: 173491
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Fig. S5. (related to Fig. 4) Depleting the zygotic Ascl1a using a splice blocking MO (spMO)
(A). A schematic presentation of ascl1a gene containing a single intron of 615 bp long in the 3’UTR.
The Ascl1a splice blocking MO (spMO) recognizes the donor sites. The unspliced mRNA is predicted
to yield a PCR product of 1100 bp long. (B). PCR verification of the primers designed for assessing
the effects of Ascl1a spMO using genomic DNA template or cDNA templates made from oocytes and
embryos at different developmental stages. (C). RT-PCR results showing that injection Ascl1a spMO
markedly increased the unspliced mRNA in stage 16 embryos and therefore the PCR products of
1100bp long. (D). cMO and Ascl1a spMO injected embryos at different stages of development. (E).
qPCR detection of tubb2b expression in Ascl1a morphants at stage 18. Values are mean ± s.d.
*P<0.05. Image published in: Gao L et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |