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Fig. S6. (related to Fig. 6) Ascl1 interacts with HDAC1 (A). Optimization of the PCR cycle number for mesendoderm genes. (B-D) RT-PCR results showing the effects of HDACis at the indicated doses on mesendoderm gene expression. (E) Bisection view of embryos showing that the expression of mixer and sox17α was inhibited by Ascl1 overexpression and rescued by the application of MS-275 (1 μM) or VP (2 mM). (F) HDAC1-6MT and HA-ASCL1 mutants were used to confirm the association between Ascl1 and HDAC1 as detected under experimental conditions and shown in Fig. 6D. Image published in: Gao L et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue mixer.L tMixer X. laevis Throughout NF stage 10.5 endomesoderm sox17a.L sox17 alpha, sox17-alpha, Sox17alpha, tSox17alpha, xsox17, Xsox17-alpha, xsox17a, Xsox17alpha, xSox17alpha1, xSox17alpha2 X. laevis Throughout NF stage 10.5 endomesoderm Image source: Published Larger Image Printer Friendly View

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