XB-IMG-173493
Xenbase Image ID: 173493
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Fig. S7. (related to Fig. 6) Depleting HDAC1 using two non-overlapping MOs
(A). Sequence information for the 5’UTR proximal to the ATG and the sequences recognized by two
non-overlapping HDAC1 MOs (HDAC1 MO1 and MO2), and the western blots showing HDAC1
MO1 and MO2 blocked the translation of the reporter mRNA with wild type 5’UTR, but not the
reporter with a mutated 5’UTR. (B) Information related to HDAC2 MO and its translation-blocking
efficacy assessed through a reporter assay. (C) qPCR detection of gene expression of HDAC1 or
HDAC2 morphants at stage 10.5. Values are mean ± s.d. * indicates p<0.05; ** indicates p<0.01 by
Student’s t-test. Image published in: Gao L et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |