Xenbase Image ID: 173551

Figure 5 From: Ras-dva small GTPases lost during evolution of amniotes regulate regeneration in anamniotes. Down-regulation of xRas-dva1 and xRas-dva2 genes functioning results in abnormal regeneration of tadpole’s tails. (A–E) The transmitted light and fluorescent images of regenerated tails of 8 dpa tadpoles injected by solution of control MO (A), mismatched mis-xRas-dva MO (B), xRas-dva1 MO (C), xRas-dva2 MO (D) or xRas-dva1 MO + xRas-dva2 MO (E) mixed with fluorescent tracer FLD. The red dashed line indicates the amputation level. Scale bar 0,5 mm. (F) Quantification of abnormal regenerates percentage of tadpoles, injected by different MO solutions or by the “rescue” mixtures of xRas-dva1 MO + xRas-dva1 RNA, xRas-dva2 MO + xRas-dva2 RNA. Error bars indicate SD. Statistical significance was determined with paired sample t-test, the results are statistically significant, p < 0,001 (asterisk). (G) qRT-PCR analysis of expression levels changes of regeneration markers Fgf20, Msx1b and also Xenopus Ag1 homolog, xAg2, during the regeneration process (at 0 and 2 dpa) in amputated tails of tadpoles injected by control, xRas-dva1 or xRas-dva2 MO solution. The value of normalized PCR signal in the 0 dpa sample, harvested immediately after amputation, was taken as an arbitrary unit (a.u.) in each series. Data are represented as mean ± SD, t-test, p < 0,05 (asterisk). Image published in: Ivanova AS et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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