Xenbase Image ID: 173557

Figure S3. Analysis of xRas-dva genes activity in tails and hindlimbs during non-regenerating stages. (A and B) Expression pattern of xRas-dva1 and xRas-dva2 at 0, 1 and 2 days post amputation at refractory 46 stage revealed by in situ hybridization. At 0dpa we detected Ras-dva1 and Ras-dva2 expression in notochord. Strong activity of gene is seen in wound epithelium and notochord tip cells at 1 dpa, but by 2 dpa expression level decreases. Nc - notochord, nct - notochord tip cells, we - wound epithelium. Dashed red line – amputation level. Dorsal to the right, distal to the top (C, D, E) The in vivo imaging of proRas-dva1-EGFP transgenic tadpoles after amputation in refractory period. (C) At 0dpa we detected EGFP expression only in notochord (nc). (D and E) Strong xRas-dva1 promoter activation was determined in wound epithelium (yellow arrowheads) and notochord tip cells at 1 dpa, but by 2 dpa expression level decreased and presented only in thin wound epithelium layer and notochord proximal to the amputation line. Dorsal to the top, distal to the left. We – wound epithelium, nc – notochord, nct – notochord tip cells. (F) The qRT-PCR analysis of xRas-dva and Fgf8 expression dynamics in hindlimbs stumps after amputation at prometamorphic stages (57 stage and later). As can be seen only xRas-dva1 is slightly activated at 1dpa. Image published in: Ivanova AS et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue ras-dva1.L LOC398404, ras-dva X. laevis Sometime during NF stage 40 to NF stage 66 wound epidermis notochord ras-dva2.S LOC733456 X. laevis Sometime during NF stage 40 to NF stage 66 notochord wound epidermis Image source: Published Larger Image Printer Friendly View

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