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Figure S7. Histological analysis of tadpoles’ tails at 1, 2 dpa upon xRas-dva1 or xRas-dva2 knock-down. (A-C) Hematoxylin staining of sagittal cryosections of 1dpa tails of tadpoles injected by control vivoMO (A) or by xRas-dva1 vMO (B) or xRas-dva2 vMO (C) tadpoles. Knock-down of Ras-dva genes results in problems in wound epithelium formation. (D-E) Hematoxylin staining of sagittal cryosections of 2dpa tails of control (D) or injected by xRas-dva1 MO (E) or xRas-dva2 MO (F) tadpoles. Down-regulation of Ras-dva genes result in reduction of spinal cord and notochord regrowth and lower density of blastemal cells in contrast to control regeneration. Dashed black line indicates the amputation level. Scale bar 0,1 mm. Bc – blastemal cells, na – neural ampule, nc – notochord, sc – spinal cord, we – wound epithelium. Cryosections width 18μm. Image published in: Ivanova AS et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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