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Figure S8. Apoptosis profiles in amputated tail of tadpoles with normal and inhibited Ras-dva1 or 2 functioning at 1dpa. (A-C) Transmitted light and fluorescent images of distal areas of 1dpa tail of the tadpole, developed from embryo injected by control MO (A) or xRas-dva1 MO (B) or xRas-dva2 MO (C). The bright green dots on fluorescent images indicate the apoptotic nuclei after TUNEL assay. Scale bar 0,25 mm. Distal to the right, dorsal to the top. Red dashed line marks the amputation level. (D) The density value of the numbers of apoptotic TUNEL-labeled nuclei in the tadpoles tails injected by control or Ras-dva-specific MO was calculated using ImageJ software. Data are represented as mean ±SD. N – number of tails used in the assay in three independent experiments. Image published in: Ivanova AS et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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