Xenbase Image ID: 173563

Figure S9. Detection of injected ectopic xRas-dva1 and xRas-dva2 mRNAs in refractory tadpoles tails after amputation. (A and A’) Transmitted light and fluorescent tail images of tadpoles developed from embryos injected by EGFP-xRas-dva1 mRNA and amputated in refractory period (st. 46). Fluorescent signals in regenerating refractory tail in fins and tip demonstrate that injected mRNA is still functions. Scale bar 2mm. (B and C) In situ hybridization staining of control refractory tadpole’s tails at 2dpa for xRas-dva1 and xRas-dva2 expression show weak signal in the tail tip. (D and E) In situ hybridization staining of refractory tadpole’s tails developed from embryos, which were injected at 2-4 cell stage by synthetic xRas-dva1 or xRas-dva2 mRNA, demonstrate xRas-dva1 (D) or xRas-dva2 (E) ectopic mRNA presence in tail tip, fins and muscles (yellow arrowheads) at 2dpa. Image published in: Ivanova AS et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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