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Xenbase Image ID: 173568

Figure 1. The peripherin targeting signal is contained within a ten amino acid residue stretch. Panels show confocal images of transgenic frog retinas expressing the reporter construct YFP-xRhoCTδ5 (green) fused to the fragments of the peripherin C-terminus illustrated in cartoons above the corresponding panels. Partial mislocalization of several constructs from rod outer segments is marked by white arrowheads. (A) The YFP-xRhoCTδ5 reporter. (B) The reporter fused to xPer 317–336. (C) The reporter fused to xPer 317–327. (D) The reporter fused to xPer 327–336. (E–G) The reporter fused to xPer 317–336 sequences containing polyalanine amino acid substitutions indicated below the panels. Abbreviations are: OS – outer segment, IS – inner segment, N – nuclei, ST – synaptic termini. The nuclei (blue) are stained with Hoechst; scale bar: 5 µm. (H) A schematic of a frog rod photoreceptor illustrating its principle compartments.

Image published in: Salinas RY et al. (2013)

Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder.

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