XB-IMG-173687
Xenbase Image ID: 173687
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Figure 2. Subcellular localisation of FAM183B. (A) Co-localisation of mouse FAM183b-GFP with centrin4
in stage 33 Xenopus embryos. (B) Detection of C- and N-terminally Flag-tagged FAM183b in mIMCD3 cells
by indirect immunofuorescence showing partial overlap with γ-tubulin. (C) Co-IP of tagged FAM183b and
NUP93, indicating interaction. Red asterisks: co-IP; black asterisk: anti-Flag light chain detected by secondary
antibody; black arrowhead: FAM183b at the expected apparent molecular weight; red arrowheads: background
band detected in transfected CHO cells and IPs. Full size Western blots are shown in Supplementary Figure S2.
(D) Co-localisation of endogenous NUP93 with CEP63 and γ-tubulin at centrosomes. Scale bars: Ba–d,a’–d’,
Da,b,a’,b’=10μm. Image published in: Beckers A et al. (2018) © The Author(s) 2018. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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