Xenbase Image ID: 173820

Figure 4. Wdr5 Is Essential for Apical Expansion and Actin Assembly in Ciliated Cells (A) The four steps of ciliogenesis in MCCs with emphasis on the role of WDR5 during the third step of MCC formation. (B) Xenopus epidermal MCCs marked with anti-acetylated α-tubulin (green, cilia) and utrophin-GFP (magenta). Cyan and white arrowheads show ciliated cells with apical enrichment and loss of actin, respectively. (C and D) Apical area of MCCs (C) and non-MCCs (D) in controls and wdr5 MO-injected embryos. n = number of MCCs. (E) The model representing actin-dependent apical surface expansion in MCCs. (F–H) Quantitative analysis of apical enrichment of actin in MCCs and neighboring non-MCCs in controls and (F) wdr5 MO- or (G) wdr5 CRISPR-injected embryos. Actin enrichment was quantified (H) using a line scan (white line) spanning MCC and neighboring non-MCC. Actin was stained using phalloidin. n = number of MCCs. ★ and ★★★ indicate statistical significance at p < 0.05 and p < 0.0005. Data are represented as mean ± SEM. See also Figures S5 and S6. Image published in: Kulkarni SS et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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