Xenbase Image ID: 173822

Figure 6. WDR5-Basal Body Complex Interacts with F-Actin during Apical Expansion (A) A montage of Xenopus epidermal MCC undergoing apical expansion over 21 min. WDR5 marked with WDR5-GFP localizes to the apical membrane as MCC expands. (B and D) A montage of Xenopus epidermal MCC expressing WDR5-GFP and centrin-RFP early during MCC expansion process. Basal bodies (centrin-RFP) and WDR5 appear to co-localize deep in the cytoplasm. (C) Schematic showing optical sections of an MCC to examine WDR5 and basal body co-localization in (B) and (D). Optical sections 1–4 correspond to the optical sections in (B) and (D). Scale bar represents 5 μM. (E) A montage of Xenopus epidermal MCC undergoing apical expansion over 9 min. WDR5 (WDR5-GFP) interacts with F-actin (utrophin-RFP) at the apical surface as MCC expands. The region in a white square is magnified to show F-actin organizing around WDR5 (note white arrowheads). See also Videos S2, S3, and S4. Image published in: Kulkarni SS et al. (2018) Copyright © 2018. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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