Xenbase Image ID: 173947

Fig. 7 Assessing the retention of pluripotency during neural crest development. (A) Contrasting models of neural crest development. Model 1: neural crest emerges from an intermediate population that retains blastula pluripotency (29). Model 2: neural crest emerges from ectoderm and reactivates pluripotency. (B) Ancestors inferred from scRNA-seq support model 2, where neural crest derives from neural cells at the neural plate border. (C) Single-cell visualization (SPRING) of neuroectoderm, non-neural ectoderm, and neural crest also indicates that neural crest derives from the neural plate border. (D) Neural crest differentiation involves hundreds of >3-fold dynamic marker genes. (E) At stage 11, the shared pluripotency circuit proposed by Buitrago-Delgado et al. (30) - foxd3, c-myc (myca), id3, tfap2a, ventx2.1, ets1, and snai1 and pou3f5.2 - is expressed broadly in nonpluripotent cells. Score shows normalized aggregate expression; see fig. S18 for individual genes. Image published in: Briggs JA et al. (2018) Copyright © 2018. Reprinted with permission from AAAS. Readers may view, browse, and/or download this image for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. Larger Image Printer Friendly View

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