Xenbase Image ID: 174003

Figure S3: Early markers of dorso-ventral development and cilia cell fate. (a) In situ hybridization for early developmental markers at stage 10-11 Xenopus embryos in uninjected controls (top row) and wdr5 morphants (bottom row). All vegetal views with dorsal to the left (at stage 10-11). (b) In situ hybridization for sox2 at stage 14, with an anterior view with dorsal to the top. (c) In situ hybridization for dand5 and xnr1 in the Xenopus LRO at stage 16. Margin of the LRO is indicated by dashed white lines. (d) In situ hybridization for shh and lefty at stage 16, with an anterior view with dorsal to the top. (e) In situ hybridization for rfx2 and dnah9 in the Xenopus LRO at stage 16. Margin of the LRO is indicated by dashed white lines. (f) Quantification of in situ hybridization for the midline marker dand5, xnr1, shh, lefty, rfx2 and dnah9. Abnormal is either reduced or absent expression compared to wildtype expression (normal). Experiments were repeated 2-3 times. ‘n’ = number of embryos. Image published in: Kulkarni SS and Khokha MK (2018) Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue foxj1 foxj1-a, foxj1-b, hfh-4, hfh4, xfoxj1 X. tropicalis Sometime during NF stage 10 to NF stage 11 dorso-lateral marginal zone wnt8a wnt-8, wnt8, Xwnt-8, Xwnt8 X. tropicalis Sometime during NF stage 10 to NF stage 11 ventro-lateral marginal zone dkk1 dickkopf-1, Dickkopf1, dkk, dkk-1, Xdkk-1, xdkk1 X. tropicalis Sometime during NF stage 10 to NF stage 11 dorsal marginal zone nodal3.1 nr3, Xnr-3, Xnr3 X. tropicalis Sometime during NF stage 10 to NF stage 11 dorsal marginal zone upper blastopore lip otx2 otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. tropicalis Sometime during NF stage 10 to NF stage 11 upper blastopore lip dorsal marginal zone myf5 myf-5, Xmyf-5, Xmyf5 X. tropicalis Sometime during NF stage 10 to NF stage 11 dorso-lateral marginal zone sox2 anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. tropicalis Throughout NF stage 14 neural plate preplacodal ectoderm dand5 51-B6, cer2, cerl2, charon, cktsf1b3, coco, crl2, dante, grem3, S10-51-B6, xCoco X. tropicalis Throughout NF stage 16 gastrocoel roof plate nodal1 nodal, nodal-related 1, nr1, nr1-a, spaw, Xnr-1, Xnr1 X. tropicalis Throughout NF stage 16 gastrocoel roof plate shh hedgehog, vhh-1, X-SHH, Xhh, xshh X. tropicalis Throughout NF stage 16 notochord neural groove lefty antivin, atv, leftb, lefty-1, lefty-a, lefty-b, lefty1, leftyb, XATV, Xlefty X. tropicalis Throughout NF stage 16 notochord rfx2 X. tropicalis Throughout NF stage 16 left-right organizer primordium dnah9 dnah9-A, L-R Dynein, Left-Right Dynein, LRD X. tropicalis Throughout NF stage 16 left-right organizer primordium Image source: Published Larger Image Printer Friendly View

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