Xenbase Image ID: 174124

Figure S5. Effects of Eya1 knockdown on NPB markers and other ectodermal TFs. A-J: Expression of PPE (Six1, Sox3), NC (FoxD3), neural plate (Sox3) markers and early ectodermal TFs in dorsal views of neural plate stage Xenopus embryos after injection of Eya1 MO1 + MO2. Anterior is to the bottom. Control side is shown on the left and injected side on the right (as indicated by blue LacZ staining). Arrowheads indicate expression domains on the control side. Reductions (arrows) and increased or ectopic expression domains (asterisks) in the neural (green) and non-neural ectoderm (orange) compared with the control side (arrowheads) are indicated. Green lines indicate broadening of the neural plate and lateral displacement of NPB markers on the injected side (bright green) versus control side (dark green). Insets show alternative phenotypes. K: Summary of regulatory interactions. Arrows indicate requirement of Eya1 for expression of TFs (reduction after Eya1 knockdown). Bars indicate requirement of Eya1 for restriction of expression of TFs (increase after Eya1 knockdown). Faint colors indicate less frequent phenotypes. See Additional file 1: Table S8 for numbers. (PDF 2013 kb) Image published in: Maharana SK and Schlosser G (2018) © Schlosser et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue six1.L XSix1 X. laevis Sometime during NF stage 13 to NF stage 21 preplacodal ectoderm foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Sometime during NF stage 13 to NF stage 21 neuroectoderm neural crest sox3.L Sry, Xlsox3, xSox3, Xtsox3 X. laevis Sometime during NF stage 13 to NF stage 21 neural plate preplacodal ectoderm zic1.L odd-paired-like, opl, Zic-r1, Zic-related-1 X. laevis Sometime during NF stage 13 to NF stage 21 neuroectoderm neural plate neural crest pax3.L cdhs, hup2, pax-3, pax3-a, pax3-b, Waardenburg syndrome 1, ws1, xpax3 X. laevis Sometime during NF stage 13 to NF stage 21 neural plate border neuroectoderm tfap2a.L ap2, AP2a, AP2alpha, tfap2a-a, tfap2a-b, tfap2alpha, XAP-2, xap2 X. laevis Sometime during NF stage 13 to NF stage 21 neuroectoderm neural crest neural plate border non-neural ectoderm msx1.L hox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1 X. laevis Sometime during NF stage 13 to NF stage 21 neural plate border anterior placodal area foxi4.2.L foxi1a, foxi4.2-a, foxi4.2-b, foxia, XFD2, Xfoxi1, Xfoxi1a, xlFox1a X. laevis Sometime during NF stage 13 to NF stage 21 preplacodal ectoderm dlx3.L ai4, dlx3-a, dlx3-b, tdo, Xdll-2, xdlx3 X. laevis Sometime during NF stage 13 to NF stage 21 non-neural ectoderm gata2.L GATA-2, xGATA-2, xgata2 X. laevis Sometime during NF stage 13 to NF stage 21 non-neural ectoderm Image source: Published Larger Image Printer Friendly View

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