Xenbase Image ID: 174148

Figure 2. pkd2 Is Required for LR Asymmetry in Xenopus, Independently of dand5 (A) Putative sensory function of Polycystin-2 during symmetry breakage downstream of GRP-flow. (B–D) Dose-dependent loss of left-sided pitx2c expression following Pkd2-MO targeting to the GRP. Black arrowhead indicates approximate location of present or absent pitx2c expression on both sides of the embryo. White arrow highlights expression of pitx2c around the eye as proof of working ISH. (E) Model of hierarchy of the flow sensing module and possible role of pkd2 on the lateral side of the LRO downstream of flow and upstream of Dand5. (F) Epistatic single and double knockdown experiments, performed on the left or right side, as indicated. Differences in expected and experimental outcome highlighted by red significance. See text for details. See also Figure S2. **p < 0.01, ***p < 0.001 in all Figures. Numbers in parentheses indicate the number of embryos analyzed for each condition. Image published in: Vick P et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Gene Synonyms Species Stage(s) Tissue pitx2.L pitx2-A, pitx2-a, pitx2-b, pitx2c, xpitx2, xPtx2 X. laevis Throughout NF stage 28 left lateral plate mesoderm profundus ganglion trigeminal ganglion Image source: Published Larger Image Printer Friendly View

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