XB-IMG-174188
Xenbase Image ID: 174188
|
|
Figure S2: Related to Figure 2. Lineage-specific knockdown of pkd2 reveals a left-sided dorsal
requirement of Polycystin-2 in the LRO
(A) Schematic depiction of the differentially targeted areas of the gastrocoel roof in a dorsal explant of
a stage 17 neurula embryo with the central, flow-generating population of the LRO (1. red), the lateral, sensory population of the LRO (2. green), and the surrounding endodermal cells (3. yellow) that cover the lateral plate mesoderm, which will express nodal and pitx2c on the left side after flow-dependent
symmetry breakage to govern asymmetric organogenesis.
(B) Unilateral left-sided pkd2 knockdown experiment showing dose-dependent loss of pitx2c when injected into the left dorsal marginal zone (DMZ) to target the central, flow-generating GRP. Injection into the dorso-lateral marginal zone (D-LMZ) to target the sensory part of the GRP (i.e. the lateral LRO cells) caused fewer, yet significant LR defects. Injecting the ventral marginal zone (VMZ) to target the left lateral plate mesoderm did not result in miss-expression of pitx2c. *p<0.05, **p<0.01, **p<0.001 for all panels. Image published in: Vick P et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |