Xenbase Image ID: 174191

Figure S5: Related to Figure 4. Polycystin-2 function in LR axis formation is independent of canonical Wnt signaling (A-C) Wnt-dependent double axis formation. Injection of wnt8a mRNA resulted in >75% of conjoined twinning (A, C). Co-injection of Pkd2-MO resulted in a very moderate drop of twinning rates to approx. 70% (B, C). Quantification of results (C). Arrowhead indicates induced secondary axis. (D-F) Wildtype expression of brachyury during gastrulation in control specimens (D) was reduced (E) or lost (F) after injection of Xnr3-MO into the dorsal marginal zone (DMZ), demonstrating that Xnr3 was required for dorsal bra expression. Bisected embryos in D’, E’ and F’ highlight these effects. Image published in: Vick P et al. (2018) © 2018 The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Gene Synonyms Species Stage(s) Tissue tbxt.L bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury X. laevis Sometime during NF stage 10.5 to NF stage 11 mesoderm marginal zone Image source: Published Larger Image Printer Friendly View

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