Xenbase Image ID: 174365

Figure 1 Integration of rhodopsin promoter‐GFP construct into the Xenopus genome. A: Map of the transgene construct pXOP(−5500/+41)GFP. On the mapline, the 5.5 kb Xenopus principal rod opsin promoter is shown as a solid box and the GFP coding region as an open box. Below the line are shown the probes for Southern analysis. The size marks are in kb and enzyme sites are shown: X, XhoI; B, BamHI; P, PstI; N, NotI; E, EcoRI. B: BamHI‐digested DNA from 2 month old tadpoles expressing GFP in the eye (X1, X2), control adult (A) and control tadpole (T) were analyzed by Southern blot using XOP (left) or GFP (right) probes. Arrow indicates the position of the endogenous 5.5 kb BamHI XOP fragment, arrowheads indicate major bands specific to the GFP‐expressing tadpoles and asterisks indicate non‐specific hybridizing species found only in tadpole DNA. The size markers were λ HindIII digest in kb: 23.1, 9.4, 6.6, 4.4, 2.3 and 2.0. Image published in: Knox BE et al. (1998) Copyright © 1998. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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