Xenbase Image ID: 174467

Fig. 2. The function of Xenopus laevis Siamois and Twin is blocked by the antisense morpholino oligomer reagents designed in this study. (A) The MOs for Siamois and Twin target the translation initiation site. (B) In vitro translated Siamois and Twin protein were blocked only by each specific MO. (C) Secondary axes induced by ventral injection Siamois mRNA (5 pg); 77.3% (17/22) of embryos have secondary axes with formation of complete heads. (D) Secondary axis-inducing activity by Siamois mRNA is blocked by Sia-MO (4 ng); 0% (0/25) of the embryos formed secondary axes. (E) Twn-MO (4 ng) did not block the effect of Siamois mRNA; 78.9% (15/19) of embryos had secondary axes with heads. Image published in: Ishibashi H et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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