Xenbase Image ID: 174468

Fig. 4. Dorsalization by LiCl and planar neural induction require Siamois and Twin. (A) Embryo treated with LiCl at the 32-cell stage. The average of dorso-anterior index (DAI); was 9.58 (Kao et al., 1986). (B) Embryo co-injected with Sia-MO and Twn-MO prior to LiCl treatment. The average of DAI was 5.38. (C) Control embryo for (A) and (B). In all cases 8 ng of MOs and 1 pg of mRNA were injected. (D) Phenotype of dorsal-animal injection of Sia-MO (total 4 ng) and Twn-MO (total 4 ng); 76.0% (19/25) of embryos lost head structures. (E) The phenotype of dorsal-vegetal injection of Sia-MO and Twn-MO. 7.7% (2/26) of embryos lost head structures. (F) Sibling control embryo at stage 38 for (A) and (B). (G) RT-PCR analyses of the effects of Sia-MO and Twn-MO on whole embryos at stage 9 and 11. (H) Neuroectoderm (NE) region of Keller explants used for molecular studies. (I) RT-PCR analyses of the effect of Sia-MO, Twn-MO, Chd-MO, and Chordin mRNA on the NE region shown in (H). Image published in: Ishibashi H et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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