XB-IMG-174538
Xenbase Image ID: 174538
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Figure 1. Fgfr4 F0 CRISPR editing disrupts LR development. (A,B)
fgfr4 CRISPR tadpoles display organ laterality defects. Ventral view of a stage 45 live tadpole with a cardiac L-loop (B; outlined) and inverse gut coiling (B; dashed line and red arrowhead). (C) Percentages of tadpoles with laterality defects (L-loops and inverse gut coiling) for three different CRISPRs; tadpoles with L-loops and inverse gut coils were scored; only tadpoles with inverse but otherwise intact gut coiling were considered; animals with completely uncoiled guts were scored as normal, as this phenotype occurs in the control population as well. Tadpoles with both cardiac and gut looping defects were only counted once in this analysis. (D)
Pitx2c expression in the LPM of tailbud stage animals (stage 28); red arrowhead indicates absent expression. (E) Percentages of stage 28 animals with different pitx2c phenotypes; ****p < 0.0001, ***p < 0.001. Image published in: Sempou E et al. (2018) Copyright © 2018 Sempou, Lakhani, Amalraj and Khokha. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
Image source: Published
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