Xenbase Image ID: 174540

Figure 3. GRP morphology and identity are altered in fgfr4 CRISPR embryos. (A–D) GRPs of fgfr4 CRISPR animals are morphologically distinct, as shown by phalloidin (actin) and anti-acetylated tubulin (cilia) stain; phenotypes ranging from mild (B) to severe (C,D), depending on loss of small mesodermal ciliated cells. (E–H) Higher magnification of GRPs shows loss of ciliated GRP area in fgfr4 CRISPR embryos (G, H). (I–K) The pre-somitic, myoD positive portion of the GRP (outlined) is drastically reduced in fgfr4 CRISPR embryos, even in embryos in which the overall GRP morphology is preserved (J). (L) Quantification of total GRP area, defined morphologically by small, ciliated cells, is reduced in fgfr4 CRISPR embryos. (M) The myoD positive area of the GRP, normalized to total GRP area, is specifically reduced in fgfr4 CRISPR embryos. Scale bars in (A–D, I–K) = 40 μm, in (E–H) = 20 μm. **p < 0.01, ***p < 0.001. Image published in: Sempou E et al. (2018) Copyright © 2018 Sempou, Lakhani, Amalraj and Khokha. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tuba4b alpha tubulin, alpha-tubulin, tuba4, tuba4a X. tropicalis Throughout NF stage 17 cilium left-right organizer myod1 mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD X. tropicalis Throughout NF stage 17 presomitic mesoderm left-right organizer gastrocoel roof plate actb beta actin, beta-actin, g-actin X. tropicalis Throughout NF stage 17 left-right organizer gastrocoel roof plate Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + fgfr4 CRISPR + NF17 (immunohistochemistry) Fig. 3 A-H, L, M Anatomical Phenotype abnormal gastrocoel roof plate morphology abnormal left-right organizer morphology abnormally decreased number of cilium in the left-right organizer Disease congenital heart disease visceral heterotaxy Xtr Wt + fgfr4 CRISPR + NF17 (immunohistochemistry) Fig. 3 I-K Anatomical Phenotype abnormal gastrocoel roof plate morphology abnormal left-right organizer morphology decreased size of the presomitic mesoderm Disease congenital heart disease visceral heterotaxy Expression Phenotype decreased amount myod1 expression in gastrocoel roof plate decreased amount myod1 expression in left-right organizer Larger Image Printer Friendly View

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